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Universidade Estadual Paulista (UNESP) / UNIGRANRIO

Principal Investigator: Professor Willian Zambuzzi

The Brazilian group has made efforts to investigate osteoblast metabolism, mainly during its adhesion and differentiation. Over the last years, they are interested to investigate the role of protein tyrosine phosphatases on different cascades of signal transduction in cancer and osteoblast metabolism. In the context of bone biology, their recent finding have shown an intricate involvement of a LMW-PTP during osteoblast differentiation in vitro by deactivating Src. Currently, their key goal is to establish a metabolic atlas of signaling proteins activated during osteoblast adhesion in order to assist the materials scientist to develop “smart” biomaterials. In addition, recent publications from the UNESP group have reported that, during the adhesion of osteoblasts onto polystyrene, there is also an intense cross-talk of signaling pathways, mainly culminating to cytoskeletal rearrangement. It is clear that diversity of affected proteins suggests that understanding the events involved in cytoskeletal organization in response to different biomaterials may yield an informed design platform/rationale for the future development of novel biomaterials. Professor Zambuzzi has relevant experience of international research collaboration with groups in The Netherlands (Prof. Maikel P. Peppelenbosch), UK (Dr. Sergio Bertazzo), USA (Prof. Paulo G. Coelho).

1. Zambuzzi WF, Coelho PG, Alves GG, Granjeiro JM. Intracellular signal transduction as a factor in the development of 'smart' biomaterials for bone tissue engineering. Biotechnol Bioeng. 2011 Feb 23. doi: 10.1002/bit.23117. [Epub ahead of print]
2. Milani R, Ferreira CV, Granjeiro JM, Paredes-Gamero EJ, Silva RA, Justo GZ, Nader HB, Galembeck E, Peppelenbosch MP, Aoyama H, Zambuzzi WF. Phosphoproteome reveals an atlas of protein signaling networks during osteoblast adhesion. J Cell Biochem. 2010 Apr 1;109(5):957-66.
3. Zambuzzi WF, Milani R, Teti A. Expanding the role of Src and protein-tyrosine phosphatases balance in modulating osteoblast metabolism: lessons from mice. Biochimie. 2010 Apr;92(4):327-32. Epub 2010 Jan 18. Review.
4. Zambuzzi WF, Bruni-Cardoso A, Granjeiro JM, Peppelenbosch MP, de Carvalho HF, Aoyama H, Ferreira CV. On the road to understanding of the osteoblast adhesion: cytoskeleton organization is rearranged by distinct signaling pathways. J Cell Biochem. 2009 Sep 1;108(1):134-44.
5. Zambuzzi WF, Granjeiro JM, Parikh K, Yuvaraj S, Peppelenbosch MP, Ferreira CV. Modulation of Src activity by low molecular weight protein tyrosine phosphatase during osteoblast differentiation. Cell Physiol Biochem. 2008;22(5-6):497-506.

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